Part:BBa_K3610003:Design
BAK1 Ectodomain from Arabidopsis thaliana
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 576
Illegal PstI site found at 621 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 576
Illegal PstI site found at 621 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 576
Illegal PstI site found at 621 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 576
Illegal PstI site found at 621 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The juxtamembrane domain must be included in the sequence since the interaction with other plant receptors has shown to be weaker without this domain.
The native signal peptide was removed between bp 77 and 78. The kinase domain was cut off between bp 863 and 865.
Source
Cyril Zipfel Lab at UZH, Switzerland
References
Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.
Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.
Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.
Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.
Zhou, Qi; Liu, Jun; Wang, Jingyi; Chen, Sufen; Chen, Lijuan; Wang, Jinfa et al. (2020): The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling. In: Journal of integrative plant biology 62 (5), S. 556–562. DOI: 10.1111/jipb.12847.